【IL 10】重组人白介素-10
产品报价
¥800/2μg ¥2080/10μg ¥74880/1mg
背景资料
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
产品描述
人白介素-10重组蛋白是在大肠杆菌中重组表达的,由两条共价结合的多肽链组成;每条链包含有161个氨基酸,分子量为18606.29Da。该人白介素-10重组蛋白最终由标准的色谱技术纯化出。
来源宿主
大肠杆菌中重组表达
序列
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.
溶解建议
提供的人白介素-10(IL 10)产品为过滤灭菌的白色冻干粉末;该冻干粉末是从含1mg/ml人白介素-10(IL 10)的溶液(不含添加剂)中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100μg/ml,后继实验过程中可根据实验要求进一步稀释。
保存建议
人白介素-10(IL 10)在室温可保存3周,或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延长保存时间,建议分装(加载体蛋白如0.1% HSA或 BSA)后置于-18℃冻存。请避免反复冻融。